Bromothymol Blue

CAS Number:  76-59-5
Molecular Weight: 624.38
MDL number: MFCD00005872
EC Index Number: 200-971-2

General description

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:

• as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
• for the demonstration of fungal hyphae within plant roots
• in the assessment of the soluble acidity within single rice grains
• use in an agar gel medium for enumeration of Bacillus cereus
• as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.