Bromocresol Green

CAS Number: 76-60-8
EC Number: 200-972-8
Molecular Weight: 698.01
PubChem Substance ID: 24847166

General description

Bromocresol Green is a synthetic triphenylmethane dye that is anionic in nature. At pH 3.8-5.4, it changes color from yellow to blue-green, and is therefore, commonly used as a pH indicator.[1]


Bromocresol green serves as a pH indicator in biology and medicinal experiments.

  • It is used widely as a vital stain to study blood-brain permeability.
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.[2]
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.[3]
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
  • It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
grade ACS reagent
Quality Level uality Level 200
Assay ≥95%
form powder
composition Dye content, 95%
visual transition interva 3.8-5.4, yellow to blue(passes test)
mp 225 °C (dec.) (lit.)
Amax 423 nm
application(s) diagnostic assay manufacturing