Bromocresol Green
CAS Number: 76-60-8
EC Number: 200-972-8
Molecular Weight: 698.01
PubChem Substance ID: 24847166
General description
Bromocresol Green is a synthetic triphenylmethane dye that is anionic in nature. At pH 3.8-5.4, it changes color from yellow to blue-green, and is therefore, commonly used as a pH indicator.[1]
Application
Bromocresol green serves as a pH indicator in biology and medicinal experiments.
- It is used widely as a vital stain to study blood-brain permeability.
- It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.[2]
- It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.[3]
- It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
- It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
| grade | ACS reagent |
| Quality Level | uality Level 200 |
| Assay | ≥95% |
| form | powder |
| composition | Dye content, 95% |
| visual transition interva | 3.8-5.4, yellow to blue(passes test) |
| mp | 225 °C (dec.) (lit.) |
| Amax | 423 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
