Neutral Red
CAS Number: 553-24-2
Molecular Weight: 288.78
PubChem Substance ID: 24897804
EC Number: 209-035-8
General description
Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.
Application
Neutral red has multiple staining applications that include its use as a nuclear stain, counterstain in the Gram procedure, either alone, or as a component of the Twort stain (a fast green-neutral red mixture). Neutral red staining is also a viability assessment method used for the detection of viable yeast cells in dandruff samples and of mussel larvae. Neutral red is also recommended for use in viability assessment as it is selectively absorbed by lysosomes and other acidic organelles in cells. It is also used for supravital staining of living blood cells.
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Biochem/physiol Actions
Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.
Quality
Purified
| Form | powder |
| Quality Level | 200 |
| composition | Dye content, ≥90% |
| technique(s) | titration: suitable |
| pH | 6.8-8.0, red to yellow |
| mp | 290 °C (dec.) (lit.) |
| solubility | water: 10 mg/mL, dark red |
| application(s) | diagnostic assay manufacturing hematology histology |
| storage temp.
|
2-8°C |
| SMILES string | Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1 |
