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Bromothymol Blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acid in a liquid. It is typically sold in solid form as the sodium salt of the acid indicator.
| CAS No. | : | 76 – 59 – 5 |
| Chemical Formula | : | C27H28Br2O5S |
| Molecular Weight | : | 624.40 |
| Melting Point | : | 200 – 202° c |
| Dye Content | : | ~95% |
| Solution Clarity | : | To pass |
| Transition Range | : | pH 5.8 – 7.6 Yellow – Blue |
| Absorption Max (pH 5.8) | : | 430 – 435 nm |
| Absorption Max (pH 7.6) | : | 615 – 618 nm |
| Absorptivity (1%/1cm) pH 5.8 | : | 260 – 300 |
| Absorptivity (1%/1cm) pH 7.6 | : | 470 – 520 |
| Loss On Drying (110° c) | : | < 3% |
Rose bengal is a stain with a pink color. Its disodium salt form is commonly available in eye drops as a diagnostic agent when there is suspected damage to conjunctival and corneal cells. It is also used in laboratory settings, including the preparation of Foraminifera for microscopic analysis and suppression of bacterial growth in several microbiological media. The clinical applications of rose bengal as injectable formulation under the name PV-10 in melanoma, breast cancer and skin conditions such as eczema and psoriasis are being investigated in clinical trials.
Bromocresol Green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualisation of the compounds with functional groups whose pKa is below 5.0.
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