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Cresol Red is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) electrophoresis. In agarose, Cresol Red runs with an apparent molecular size of approx. 125 bp DNA. Cresol Red does not inhibit Taq polymerase. Recently Cresol Red has been used as a pH sensitive indicator dye in nanoengineered optical urea biosensors films and hetero-core structured fiber optic pH sensor.
| grade | indicator grade |
| InChI Key | OBRMNDMBJQTZHV-UHFFFAOYSA |
| composition | Dye content, 95% |
| useful pH range | 1.8 – 2.0, orange to yellow (acid) |
| 7.0 – 8.8, yellow to violet (alkaline) | |
| pH range | 7.2 – 8.8, yellow to red |
| absorption | λmax 367 nm (2nd) |
| λmax 570 nm |
Thymolphthalien is an acid-base indicator which is colorless in acidic form and deep blue in basic form. Acidic form retains hydrogen on each hydroxyl group. It is suitable in preparing disappearing ink.
Thymolphthalein was suitable as indicator during titration of hyamine hydroxide.
Bromothymol Blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acid in a liquid. It is typically sold in solid form as the sodium salt of the acid indicator.
2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke.
2,3,5-Triphenyltetrazolium chloride has been used to stain heart tissue to measure the extent of acute lesion. It has also been used to stain brain tissue to check the size of infarct area.
Bromocresol Green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualisation of the compounds with functional groups whose pKa is below 5.0.
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