Janus Green B

CAS Number: 2869-83-2
Molecular Weight: 511.06
PubChem Substance ID: 24852018
EC Number: 220-695-6
Colour Index Number: 11050



Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.[1][2][3]

Biochem/physiol Actions

Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder.[4] Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells.[5] It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.[4]

grade certified by the Biological Stain Commission
Quality Level 200
form powder
composition Dye content, 65%
Amax 395 nm
660 nm (2nd
E (extinction coefficient) ≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L
application(s)| diagnostic assay manufacturing
storage temp. 2-8°C
SMILES string [Cl-].CCN(CC)c1ccc2nc3ccc(cc3[n+](-c4ccccc4)c2c1)\N=N\c5ccc(cc5)N(C)C
InChi 1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1