Bromophenol Blue
Bromophenol Blue is an acid-base indicator since its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. Bromophenol Blue is structurally related to phenolphthalein (a popular indicator). Bromophenol Blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresisThey are used in inks, paints, and as indicators and reagents. Concentrations of Bromophenol Blue in plasma, urine, and bile were determined spectrophotometrically after intravenous bolus injections and infusions in rats. Bromophenol Blue (BPB) is the most widely used stain in gel electrophoresis for tracing the migration of samples on electrophoretic gels.
| Specifications | Parameters |
| C.A.S. No. | 115-39-9 |
| Molecular Weight | 669.99g/mole |
| Solubility 0.1% (Ethanol) | Clear solution |
| Transition Range | pH 3.1 – pH 4.6 |
| Greenish yellow-Blue violet | |
| Absorption maximum (pH 3.1) | 434 – 439nm |
| Absorptivity (1%/ 1cm at pH 3.1) | 350 – 385 |
| Absorption maximum (pH 4.6) | 590 – 593nm |
| Absorptivity (1%/ 1cm at pH 4.6) | 940 – 1000 |
| Dye content (by Spectrophotometry) | > 95% |
| Loss On Drying at 110 degrees C (1hr) | <1% |
